Skeletonises the mask image and extracts a set of lines connecting node points on the skeleton. Then produce a set of lines connecting node points on the skeleton.Īnalyses an image using a given mask. Skeletonise (reduce to single pixel lines) a mask image. All pixels outside the mask in the target image will be set to zero. Requires the imagescience.jar file from the FeatureJ package.Ĭreate a mask from a source image using the Mask Creater and apply it to a target image. Optionally allows: the edge lines to be pruned to leave only closed loops and filling of loops to create a mask. Edges are defined where the gradient is steepest. The plugin can operate on stacks and optionally can extract a specified frame, slice or channel to create the mask.Ĭreate an edge mask from an image. Threshold: perform thresholding (z-stack planes from the same time point are thresholded collectively).Use ROI: any pixel inside the marked ROI.This respects the display value for each channel Min Display Value: any pixel above the minimum displayed value (set using Image > Adjust > Brightness/Contrast).
Based on the Multi Threshold plugin developed by Yasunari Tosa the modifications allow processing 16-bit images and further output optionsĬreates a mask from an image with the following options: Thresholds an image into levels using multiple thresholds. This is a fast method that can be used within the Find Peaks algorithm
#Imagej threshold selection plus#
An additional method had been added that sets the threshold as the image average plus a factor of the standard deviation. This is a modified version of the Auto Threshold plugin developed by Gabriel Landini. Provides methods to threshold an image into foreground and background pixels.
The following tools are available: Binary Image Processing Threshold The GDSC plugins provide various utility tools for image analysis.
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